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| Services >> Proteomics |
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| E. coli Expression System |
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E. coli Expression System
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Protein expression R & D platform is a crucial step in the projects involved in the investigation of proteins structures and functional properties. The quality of the purified protein is an essential factor. Crnbio Recombinant Protein Expression platform Service is a complete solution for your needs, helping your organization to deal with the protein bottleneck. The service combines a variety of expression systems , across a range of scales, with a full range of purification options, providing you with the material that you require for your application. The service spans vector construction through to purification of ready to use protein and can be tailored to meet your exact requirements. Crnbio offers you the adequate customised and flexible service with advice during all the project phases at very competitive price.
- Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Crnbio offer the E. coli expression services.
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- Procedure
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1. Foreign gene design and codon optimizing
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2. Reading frame design and/or cds synthesis.
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3. Plasmid constructing.
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4. Plasmid transformation and expression strain selection.
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5. Optimizing: Incubation and induction of protein expression.
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6. Protein pufification
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7. Protein verification and modification.
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R & D Flowchart
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Reaction gra
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Service Quote and Delivery Time:
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Customer Notices
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1. Customer should ensures that the materials provided are correct (including gene templates, primers, expression vectors).
2. Customer is AOR for the mutated gene templates or expression vectors.
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References
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 Magnetic Resonance Imaging of Tumors Colonized with Bacterial Ferritin-Expressing Escherichia coli.pdf
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Reprogramming of Escherichia coli K-12 Metabolism during the Initial Phase of Transition from an Anaerobic to a Micro-Aerobic Environment.pdf |
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