Reverse transcription polymerase chain reaction (RT-PCR) is a variant of polymerase chain reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed "amplification". In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR. ALV follow the rules of MIQE to prepare the cDNA samples for qPCR.
Service Contents
1. RT-PCR test the gene expression abundance.
2. RT-PCR to test the gene expression viabilities during treatments, using β-Actin or GAPDH as control.
3. Prepare cDNAs for qPCR assay.
Service Quote
RNA Extraction( whole RNA extraction)
RT-PCR
PCR
