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| Products >> Antibodies、Peptides >> Primary antibodies |
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| Anti-GAPDH antibody [4D8] crn-0020M |
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- Product ID crn-0020M
- Product name Anti-GAPDH antibody [4D8]
Product Price 100µg ＄ 260
Immunogen full length GAPDH protein
Reacts with Chk, Dog, Fsh, Hu, Mk, Ms, Pig, Rb, Rat, Zfsh
Does not react with cow Goat
SClone number 4D8
Isotype IgG1
Purity Protein A purified
Storage buffer Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Form Lyophilized or Liquid
Concentration 1.000 mg/ml
Recommended dilutions ELISA: Use at an assay dependent dilution(1:1000-5000) .
ICC: Use at an assay dependent dilution(1:50-500).
ICC/IF: Use at an assay dependent dilution(1:100-1000).
IHC-Fr: 1:100-1000.
- WB: 1/100 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight:
- 40.2 kDa). (PubMed: 16450009)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA
glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-
3-phosphate in the presence of inorganic phosphate and nicotinamide
adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate
Background metabolism. While GAPDH has long been recognized as playing an integral
role in glycolysis, additional functions of GAPDH include acting as a uricil
DNA glycosylase, activating transcription, binding RNA and involvement in
nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH
is upregulated in liver, lung and prostate cancers. GAPDH translocates to the
nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated
in human neuro-degenerative disorders including the β-Amyloid precursor,
Huntingtin and other triplet repeat neuronal disorder proteins.
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Function |
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
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Pathway |
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. |
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Sequence similarities |
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
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Post-translational
modifications |
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. |
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Cellular localization |
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. |
 
- localization.GAPDH （4D8）. Western blot - GAPDH antibody [4D8]
- Immunofluorescence staining of methanol- Predicted band size : 40.2 kDa
fixed HeLa cells showing cytoplasmic
- localization.
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