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| Products >> Antibodies、Peptides >> Primary antibodies |
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| Anti-beta Actin antibody - Loading Control crn-0021R |
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- Product ID crn-0021R
- Product name Anti-beta Actin antibody
Product Price 100µg ＄ 260
Immunogen Synthetic peptide derived from within residues 1 - 100 of Human beta Actin.
- Reacts with Hu, Ms, Rat, Chk,, Cow, Dog, Fsh, Mk, Pig, Rb, RMk, Shp
- Does not react with Zfsh
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Raised in |
Rabbit |
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Clonality |
Polyclonal |
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Isotype |
IgG |
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Purity |
Immunogen affinity purified |
- Storage buffer Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Form Lyophilized or Liquid
Concentration 1.000 mg/ml
Recommended dilutions ELISA: Use at an assay dependent dilution(1:1000-5000) .
ICC: Use at an assay dependent dilution(1:50-500).
ICC/IF: Use at an assay dependent dilution(1:100-1000).
IHC-Fr: 1:100-1000.
- WB: 1/100 - 1/1000.
- Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
All eukaryotic cells express Actin, which often constitutes as much as 50% of
total cellular protein. Actin filaments can form both stable and labile structures
and are crucial components of microvilli and the contractile apparatus of muscle
cells. While lower eukaryotes, such as yeast, have only one Actin gene,
- Background higher eukaryotes have several isoforms encoded by a family of genes. At
least six types of Actin are present in mammalian tissues and fall into three
classes. α-Actin expression is limited to various types of muscle, whereas
β- and γ-Actin are the principle constituents of filaments in other tissues.
Members of the small GTPase family regulate the organization of the Actin
cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion,
Rac regulates Actin filament accumulation at the plasma membrane
and Cdc42 stimulates formation of filopodia.
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图1 β-Actin .Western blot analysis of β-Actin expression in Jurkat whole cell lystate (A) and mouse intestine tissue extract (B).
图2 ICC/IF image of crn-0021R stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS- Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (crn-0021R , 5µg/ml) overnight at +4°C. The secondary antibody (green) was goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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