Product ID                                     crn-0022R  

English name                              Anti-14-3-3

Product Price                               100µg   ＄ 520   
Immunogen                                conjugated synthetic peptide derived from human 14-3-3 C-terminus.
Reacts with                                  Hu, Ms, Rat

Does not react with                    

Raised in	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purity	Immunogen affinity purified

Cellular localization

Cytoplasmic

Storage buffer                               Preservative: 0.1% Sodium Azide  
                                                        Constituents: PBS, pH 7.4 
Form                                               Lyophilized or Liquid  
Concentration                              1.000 mg/ml  
Recommended dilutions          ELISA: Use at an assay dependent dilution(1:1000-5000) .
                                                        ICC: Use at an assay dependent dilution(1:50-500).
                                                        ICC/IF: Use at an assay dependent dilution(1:100-1000).
                                                        IHC-Fr: 1:100-1000.                                                    

                                                        WB: 1/100 - 1/1000.                                                      

                                                        Not yet tested in other applications.
                                                        Optimal dilutions/concentrations should be determined by the end user.
                                                       

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

                                                             

	Anti-14-3-3 antibody for Western blot (Human)