Product ID                                     crn-0023R   

Product name                              Anti-14-3-3 zeta (phospho S58)antibody

Product Price                               100µg  ＄620   
Immunogen                                 Synthetic phosphopeptide from Human 14-3-3-zeta, around                                          the phosphorylation site of serine 58 (R-S-phosphoS-W-R).

Cellular localization

Cytoplasmic

 Reacts with                                  Hu, Ms, Rat

Raised in	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purity	The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer                               Preservative: 0.1% Sodium Azide  
                                                        Constituents: PBS, pH 7.4 
Form                                               Lyophilized or Liquid  
Concentration                              1.000 mg/ml  
Recommended dilutions           ELISA: 1/40000.
                                                        ICC/IF: Use at a concentration of 1 - 5 µg/ml.
                                                        IHC-P: 1/50 - 1/100.
                                                        WB: 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). 
                                                        Not yet tested in other applications.
                                                        Optimal dilutions/concentrations should be determined by the end user.

Background

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, theta, epsilon, zeta, sigma, and nu. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzyme activity etc.

                                                       

  

                        图1                                                                                                                                 图2

图2 p-14-3-3 ζ (Ser 58): Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.