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| Products >> Antibodies、Peptides >> Primary antibodies |
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| Anti-eIF4EBP1/4EBP1 antibody crn-0024R |
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- Product ID crn-0024R
- Product name Anti-eIF4EBP1/4EBP1 antibody
- Product Price 100µg ＄600
- Immunogen Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
- Reacts with Hu, Ms, Rat, Pig
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Specificity |
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. |
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Raised in |
Rabbit |
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Clonality |
Polyclonal |
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Isotype |
IgG |
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Purity |
Immunogen affinity purified |
- Storage buffer Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Form Lyophilized or Liquid
Concentration 1.000 mg/ml
Recommended dilutions WB: 1/1000 - 1/6000. Detects a band of approximately 20-25 kDa (predicted molecular weight: 13 kDa).
. Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Function |
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
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Sequence similarities |
Belongs to the eIF4E-binding protein family. |
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Post-translational
modifications |
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. | The translation of proteins from eukaryotic mRNA is initiated by the multisubunit
complex eIF-4F, which associates with the mRNA 5' cap structure.
eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure
Background and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1,
also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1
by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1
from eIF-4E, promoting translation. A protein that is functionally related to
4E-BP1, designated 4E-BP2, also associates with eIF-4E.
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Predicted band size : 13 kDa
crn-0024R at a 1:500 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine. |
| Prev：Anti-14-3-3 zeta (ph Next：Anti-eIF4EBP1 (phosp |
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