Product ID                                     crn-0029R   

Product name                              Anti-Serotonin transporter antibody

Product Price                               100µg   $ 420   
Immunogen                                Synthetic peptide conjugated to KLH derived from human 5-HTT C-terminus.

Reacts with                                  hu, mo, rat, cow, shp, chk, dog, Gpig, pig

Raised in	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purity	Immunogen affinity purified

Storage buffer                               Preservative: 0.02% Sodium Azide

                                                        Constituents: 1% BSA, PBS, pH 7.4

Form                                               Lyophilized or Liquid  
Concentration                              1.000 mg/ml  
Recommended dilutions          ELISA: Use at an assay dependent dilution(1:1000-5000) .
                                                        ICC: Use at an assay dependent dilution(1:50-500).
                                                        ICC/IF: Use at an assay dependent dilution(1:100-1000).
                                                        IHC-Fr: 1:100-1000.                                                    

                                                        WB: 1/100 - 1/1000.                                                      

                                                        Not yet tested in other applications.
                                                        Optimal dilutions/concentrations should be determined by the end user. 

   

Background	Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Tissue specificity	Expressed in platelets (at protein level).
Sequence similarities	Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
Post-translational modifications	Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms. Phosphorylated at Ser-611, Thr-613 and Thr-616.
Cellular localization	Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.

    

 

IHC image of crn-0029R staining in rat normal brain formalin fixed paraffin embedded tissue section,  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab44520, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.