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Products >> Antibodies、Peptides >> Primary antibodies
Anti-5 Lipoxygenase (phospho S271) antibody crn-0031R
Product ID                                     crn-0031R  
Product name                              Anti-5 Lipoxygenase (phospho S271) antibody
Product Price                               100µg   $ 520   
Immunogen                                Synthesized phosphopeptide derived from human 5 Lipoxygenase around the phosphorylation site of serine 271.
Reacts with                                  rat, mo, hu

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer                               Preservative: 0.02% Sodium Azide
                                                        Constituents: 1% BSA, PBS, pH 7.4
Form                                               Lyophilized or Liquid  
Concentration                              1.000 mg/ml  
Recommended dilutions          ELISA: Use at an assay dependent dilution(1:1000-5000) .
                                                        ICC: Use at an assay dependent dilution(1:50-500).
                                                        ICC/IF: Use at an assay dependent dilution(1:100-1000).
                                                        IHC-Fr: 1:100-1000.                                                    
                                                        WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 78 kDa).   
                                                        Not yet tested in other applications.
                                                        Optimal dilutions/concentrations should be determined by the end user. 

Function 

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Pathway 

Lipid metabolism; leukotriene A4 biosynthesis.

Sequence similarities 

Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.

Post-translational
modifications 

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Cellular localization 

Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

 
All lanes : Anti-5 Lipoxygenase (phospho S271) antibody
Lane 1 : Extracts from HuvEc cells.
Lane 2 : Extracts from HuvEc cells plus phosphopeptide
 
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