产品描述 概述Recombinant Human Tryptase Alpha/Beta-1 is produced by our Mammalian expression system and the target gene encoding Ile31-Pro275 is expressed with a 6His tag at the C-terminus. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Background | Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase alpha/beta-1(TPSAB1), also known as mast cell protease 7 (MCPT7), it exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. The two Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. It may play a role in innate immunity. | | Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. | | Purity | Greater than 95% as determined by reducing SDS-PAGE. | | Reconstitution | Dissolve the lyophilized protein in distilled water. | | Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. | | Formulation_Description | Lyophilized from a 0.2 μm filtered solution of 1M NaCl, 0.05mM Heparin, 50mM sodium acetate , pH 5.0, containing 0.01% sodium azide. | | Source | Human Cells | | Accession# | Q86TM8 | | 表达系统 | Human Cells | | 种属 | Human | | Tag | C-6His |
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