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Recombinant Human TrpRS , N-6His, E.coli

产品描述

概述

Recombinant Human Tryptophan-tRNA ligase, Cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Gln471 is expressed with a 6His tag at the N-terminus.

使用说明

This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

TagN-6His
种属Human
表达系统E.coli
Accession#P23381
SourceE.coli
Formulation_DescriptionLyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
StorageLyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
ReconstitutionDissolve the lyophilized protein in distilled water.
PurityGreater than 90% as determined by reducing SDS-PAGE.
EndotoxinLess than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
BackgroundThere exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. WARS regulates ERK, Akt, eNOS activation pathway, which are related with angiogenesis, cytoskelatal reorganization and shear stess-reponsive gene expression.
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