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Recombinant Human GOT1, C-6His, E.coli

产品描述

概述

Recombinant Human Glutamate Oxaloacetate Transaminase 1 is produced by our E.coli expression system and the target gene encoding Ala2-Leu414 is expressed with a 6His tag at the C-terminus.

使用说明

This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

TagC-6His
种属Human
表达系统E.coli
Accession#P17174
SourceE.coli
Formulation_DescriptionSupplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 2mM DTT, 20% Glycerol, pH 7.5.
StorageStore at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
PurityGreater than 90% as determined by reducing SDS-PAGE.
EndotoxinLess than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
BackgroundGlutamate Oxaloacetate Transaminase 1 (GOT1) is a cytoplasmic protein. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT1 is a pyridoxal phosphate-dependent enzyme that exists in cytoplasmic and mitochondrial forms. GOT1 plays a key role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT1 involves in L-methionine salvage from methylthioadenosine, aspartate catabolic process, cellular response to insulin stimulus, polyamine metabolic process, and glucocorticoid stimulus.
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