产品描述 概述Recombinant Human Glial Cell line-derived Neurotrophic Factor Receptor Alpha 1 is produced by our Mammalian expression system and the target gene encoding Asp25-Lys429 is expressed with a Fc tag at the C-terminus. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Tag | C-Fc | | 种属 | Human | | 表达系统 | Human cells | | Accession# | P56159-2 | | Source | Human Cells | | Formulation_Description | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. | | Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. | | Reconstitution | Dissolve the lyophilized protein in distilled water. | | Purity | Greater than 90% as determined by reducing SDS-PAGE. | | Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. | | Background | Glial Cell Line-Derived Neurotrophic Factor Family Receptor α-1 (GDNFRα1) is a glycosylphosphatidylinositol (GPI) linked cell surface protein belonging to GDNF-family receptor α subtype which consists of at least four members. GFRα1and GFRα2 are the cognate co-receptor for the neurotrophic factor neurturin mediating the NRTN-induced autophosphorylation and activation of the RET tyrosine kinase receptor. Soluble GFRαs released enzymatically from the cell surface by phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFRα1, can also bind with high affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFRα1 shares 93% amino acid identity with mouse GFRα1.The expression of the various GFRαs are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFRαs in mediating the activities of the GDNF family of neurotrophic factors. |
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