产品描述 概述Recombinant Human Ribulose-Phosphate 3-Epimerase is produced by our E.coli expression system and the target gene encoding Met1-Arg228 is expressed with a 6His tag at the C-terminus. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格Tag | C-6His | 种属 | Human | 表达系统 | E.coli | Accession# | Q96AT9-1 | Source | E.coli | Formulation_Description | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 6.2. | Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. | Purity | Greater than 95% as determined by reducing SDS-PAGE. | Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. | Background | Ribulose-Phosphate 3-Epimerase (RPE) is a member of the Ribulose-Phosphate 3-Epimerase family. RPE exists as a homodimer and catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. RPE binds one divalent metal cation per subunit and contains tightly bound Fe2+ when produced in E. coli, but the physiological cofactor may be Co2+, Mn2+ or Zn2+. It has been shown that RPE participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation. |
|