产品描述 概述Recombinant Human Ethanolaminephosphotransferase 1 is produced by our E.coli expression system and the target gene encoding Met1-Pro50 is expressed with a GST tag at the N-terminus. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格Tag | N-GST | 种属 | Human | 表达系统 | E.coli | Accession# | Q9C0D9 | Source | E.coli | Formulation_Description | Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, pH 8.0. | Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. | Purity | Greater than 95% as determined by reducing SDS-PAGE. | Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. | Background | Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway. |
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