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Recombinant Rat NT5E, C-6His, Human Cells

产品描述

概述

Recombinant Rat 5'-Nucleotidase/CD73 is produced by our Mammalian expression system and the target gene encoding Trp29-Lys549 is expressed with a 6His tag at the C-terminus.

使用说明

This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

BackgroundCD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
EndotoxinLess than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
PurityGreater than 90% as determined by reducing SDS-PAGE.
StorageStore at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
Formulation_DescriptionSupplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
SourceHuman Cells
Accession#P21588
表达系统Human Cells
种属Rat
TagC-6His
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