Recombinant Rat CINC-1/CXCL1-蛋白相关-重组蛋白；-北京诺梵生物科技有限公司

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Recombinant Rat CINC-1/CXCL1

产品描述

概述

Rat CXCL1, also known as CINC-1, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.

使用说明

This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rRtCINC-1/CXCL1 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence	APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK

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