产品描述 概述Interleukin-11 (IL-11) is encoded by the IL11 gene. IL-11 is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. IL-11 is a member of the IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. IL-11 can directly stimulate the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induce megakaryocyte maturation resulting in increased platelet production. Mature murine IL-11 shares 88 % amino acid sequence identity with human IL-11. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | | Purity | > 97 % by SDS-PAGE and HPLC analyses. | | Source | Escherichia coli. | | Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine T11 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. | | Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. | | Endotoxin | Less than 1 EU/μg of rMuIL-11 as determined by LAL method. | | Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. | | AA Sequence | MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDHSLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLKTLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGSIRAAHAILGG LHLTLDWAVR GLLLLKTRL |
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