产品描述 概述Murine IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells. It promotes the anti-tumor activity of CD8+ T cells and NK cells. IL-21 elicits its effect through binding IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | | Purity | > 98 % by SDS-PAGE and HPLC analyses. | | Source | Escherichia coli. | | Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg. | | Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. | | Endotoxin | Less than 0.1 EU/μg of rMuIL-21 as determined by LAL method. | | Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. | | AA Sequence | HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS |
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