产品描述 概述Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1αand IL-1β binds to the same receptor and has similar but not identical biological properties. The mature rat IL-1β shares 90 % a.a. sequence identity with cotton rat and mouse and 65 % to 77 % with canine, human and rhesus IL-1β. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | | Purity | > 96 % by SDS-PAGE and HPLC analyses. | | Source | Escherichia coli. | | Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. | | Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. | | Endotoxin | Less than 1 EU/μg of rRtIL-1β as determined by LAL method. | | Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. | | AA Sequence | MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS |
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