Human CX3CL1/Fractalkine-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

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Human CX3CL1/Fractalkine

Human CX3CL1/Fractalkine Quantikine ELISA Kit Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4.5 hours
Sample Type & Volume Required Per Well	Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (50 uL), Urine (50 uL), Human Milk (100 uL)
Sensitivity	0.072 ng/mL
Assay Range	0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity	Natural and recombinant human Fractalkine. This assay detects all forms of human Fractalkine containing the chemokine domain
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested
Interference	No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Long Name:	CX3CL1/Fractalkine
Entrez Gene IDs:	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names:	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1. Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3. Add 50 μL of Assay Diluent to each well.

4. Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5. Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6. Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7. Aspirate and wash 5 times.

8. Add 100 μL Substrate Solution to each well.

9. Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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