产品信息 >> 试剂盒相关 >> ELISA 试剂盒;
Human Phospho-VEGFR3/Flt-4

Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA

Assay   Type

Solid   Phase Sandwich ELISA

Format

96-well   strip plate

Assay   Length

4 hours   40 minutes (after plate preparation)

Sample   Type & Volume Required Per Well

Cell   lysates (100 μL)

Sensitivity

2.15   pmol/L

Assay   Range

125.0 -   8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin   Plasma)

Specificity

Recombinant   and natural   human VEGF R3 / Flt-4 in cell lysates

 

Cross-reactivity

<   0.5% cross-reactivity observed with available related molecules.< 50%   cross-species reactivity observed with species tested

Interference

No   significant interference observed with available related molecules.

Product Summary

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human VEGF R3 / Flt-4 in cell lysates. An immobilized capture antibody specific for VEGF R3 / Flt-4 binds both phosphorylated and unphosphorylated VEGF R3 / Flt-4. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Preparation and Storage

Shipping

The   product is shipped at ambient temperature. Upon receipt, store it immediately   at the temperature recommended below.

Storage

Store   the unopened product at 2 - 8 °C. Do not use past expiration date.

Background:  VEGFR3/Flt-4

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long   Name:

Vascular   Endothelial Growth Factor Receptor 3

Entrez   Gene IDs:

2324   (Human); 14257 (Mouse)

Alternate   Names:

EC   2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41;   soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3;   Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor   receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1.     Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2.     Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3.     Add 50 μL of Assay Diluent to each well.

4.     Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5.     Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6.     Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7.     Aspirate and wash 5 times.

8.     Add 100 μL Substrate Solution to each well.

9.     Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

上一篇:Human/Mouse/Rat Phos
下一篇:Mouse M-CSF
关于我们 | 产品信息 | 技术服务 | 联系我们

     电话:400-832-8698    地址:北京怀柔雁栖开发区光织谷东区二号楼 

   备案/许可证编号京ICP备2020036913号