Human Galectin-3-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

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Human Galectin-3

Human Galectin-3 Quantikine ELISA Kit Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4.5 hours
Sample Type & Volume Required Per Well	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity	0.085 ng/mL
Assay Range	0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity	Natural and recombinant human Galectin-3. This assay cross-reacts 58% with Mouse Galectin-3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested
Interference	No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Long Name:	Galectin-3
Entrez Gene IDs:	3958 (Human); 16854 (Mouse)
Alternate Names:	AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1. Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3. Add 50 μL of Assay Diluent to each well.

4. Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5. Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6. Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7. Aspirate and wash 5 times.

8. Add 100 μL Substrate Solution to each well.

9. Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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