Human CCL13/MCP-4-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

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Human CCL13/MCP-4

Human CCL13/MCP-4 DuoSet ELISA Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4 hours 40 minutes (after plate preparation)
Sample Type & Volume Required Per Well	100 μL
Sensitivity	3.68 pg/mL
Assay Range	7.8 - 500 pg/mL (Cell Culture Supernates, Bone Extracts)
Specificity	Natural and recombinant human CCL13/MCP-4
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested
Interference	No significant interference observed with available related molecules.

Product Summary

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL13/MCP-4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL13/MCP-4

Human MCP-4, also known as CCL13, encodes a 98 amino acid (aa) precursor protein with a 23 aa hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MCP-4. Mature MCP-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins..

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name:	MCP-4Monocyte chemoattractant protein 4
Entrez Gene IDs:	6357 (Human)
Alternate Names:	C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1. Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3. Add 50 μL of Assay Diluent to each well.

4. Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5. Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6. Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7. Aspirate and wash 5 times.

8. Add 100 μL Substrate Solution to each well.

9. Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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