Human MBL-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

产品信息 >> 试剂盒相关 >> ELISA 试剂盒；

Human MBL

Human MBL Quantikine ELISA Kit Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4.5 hours
Sample Type & Volume Required Per Well	Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity	0.029 ng/mL
Assay Range	0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity	Oligomeric forms of natural and recombinant human MBL
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested
Interference	No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MBL immunoassay is a 4.5 hour solid phase ELISA designed to measure human MBL in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human MBL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MBL showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring human MBL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MBL

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which bind an array of carbohydrate patterns on pathogen surfaces. Collectin family members share several common structural features including a N-terminal, cysteine-rich domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal, C-type (Ca2+-dependent ) lectin or carbohydrate recognition domain. MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bonds. These homotrimers further associate into oligomeric structures of up to six units.

Long Name:	Mannose Binding Lectin
Entrez Gene IDs:	4153 (Human)
Alternate Names:	COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

上一篇：Human EPCR
下一篇：Mouse/Rat RGM-A