Human SLPI Quantikine ELISA Kit Summary

Product Summary

The Quantikine Human SLPI Immunoassay is a 4.5 hour solid phase ELISA designed to measure SLPI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human SLPI and antibodies raised against the recombinant factor. The kit has been shown to accurately quantitate recombinant human SLPI. Results obtained using natural human SLPI showed linear curves that were parallel to the curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SLPI.

Preparation and Storage

Background: SLPI

Secretory leukocyte protease inhibitor (SLPI) is found in various secretory fluids including parotid secretions, bronchial, nasal, cervical mucous, and seminal fluid. SLPI is a potent inhibitor of many proteolytic enzymes including the neutrophil proteases, elastase, cathepsin G, chymotrypsin, and trypsin.

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.