Mouse Fetuin A/AHSG-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

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Mouse Fetuin A/AHSG

Mouse Fetuin A/AHSG Quantikine ELISA Kit Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4.5 hours
Sample Type & Volume Required Per Well	Cell Culture Supernates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity	0.337 ng/mL
Assay Range	1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity	Natural and recombinant mouse Fetuin A
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested
Interference	No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Fetuin A Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Fetuin A levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Fetuin A and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Fetuin A accurately. Results obtained using natural mouse Fetuin A showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fetuin A.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fetuin A/AHSG

Fetuins are members of the cystatin family of proteases. Secreted by the liver, fetuins can be found at high levels in serum. Fetuin A promotes phagocytosis of apoptotic cells and plays a role in bone development, calcium homeostasis and insulin sensitivity. Fetuin B functions as an inhibitor of basic calcium phosphate precipitation.

Long Name:	alpha-2-HS Glycoprotein
Entrez Gene IDs:	197 (Human); 11625 (Mouse); 25373 (Rat)
Alternate Names:	A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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