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Human TGF-beta RII

Human TGF-beta RII DuoSet ELISA Summary

Assay   Type

Solid   Phase Sandwich ELISA

Format

96-well   strip plate

Assay   Length

4.5   hours

Sample   Type & Volume Required Per Well

100 μL

Sensitivity

/

Assay   Range

15.6 -   1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin   Plasma, Urine)

Specificity

Natural   and recombinant human TGF-beta RII

Cross-reactivity

<   0.5% cross-reactivity observed with available related molecules.Cross-species   reactivity observed with 1 or more species tested

Interference

No   significant interference observed with available related molecules.

Product Summary

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TGF-beta RII. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Preparation and Storage

Shipping

The   product is shipped at ambient temperature. Upon receipt, store it immediately   at the temperature recommended below.

Storage

Store   the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta1 and TGF-beta 3 with high affinity and TGF-beta 2 with much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction.

Long   Name:

Transforming   Growth Factor beta Receptor II

Entrez   Gene IDs:

7048   (Human); 21813 (Mouse)

Alternate   Names:

AAT3;   EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2;   tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta   receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII;   TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta   receptor type IIC; transforming growth factor, beta receptor II (70/80kDa)   isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2;   transforming growth factor, beta receptor II (70/80kDa); transforming growth   factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor   type II

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3Add 50 μL of Assay Diluent to each well.

4Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7Aspirate and wash 5 times.

8Add 100 μL Substrate Solution to each well.

9Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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