Mouse PlGF-2 Quantikine ELISA Kit Summary

Product Summary

The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.

Preparation and Storage

Background:  PlGF-2

Placenta growth factor (PlGF) is a dimeric growth factor in the PDGF/VEGF family. Alternative splicing generates at least three human PlGF isoforms PlGF 1, PlGF 2, and PlGF 3. PlGF 2 is the only isoform that contains a heparin binding region. It is expressed by villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells. PlGF competes with VEGF for binding and activating VEGF R1/Flt 1. This results in increased angiogenesis by making more VEGF available for activation of VEGF R2. PlGF 2 also exhibits heparin dependent binding of Neuropilins-1 and 2 which serve as VEGF co-receptors. It can inhibit nerve growth cone collapse and induce monocyte production of inflammatory cytokines including VEGF.

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.