Human DLL1 Quantikine ELISA Kit Summary

Product Summary

The Quantikine Human DLL1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DLL1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human DLL1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human DLL1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DLL1.

Preparation and Storage

Background: DLL1

DLL1 (Delta-like protein 1) is a transmembrane glycoprotein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription.

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.