中文名称

5-溴脱氧尿嘧啶核苷（增殖标志物）单克隆抗体

别    名

Bromodeoxyuridine; Bromodeoxyuridine;   5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker;   5-Bromo-2-deoxyUridine.  

抗体来源

Mouse

克隆类型

Monoclonal

交叉反应

Human, Rat, 

产品应用

ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） 
  not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user.

细胞定位

细胞核 

性    状

Liquid or Solid

浓    度

1mg/ml

免 疫 原

bromodeoxyuridine conjugated to   KLH: 

亚    型

IgG

纯化方法

affinity purified by Protein G

储 存 液

0.01M TBS(pH7.4) with 1% BSA, 0.03%   Proclin300 and 50% Glycerol.

保存条件

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated   freeze/thaw cycles.

PubMed

PubMed

产品介绍

Proliferation Marker
  Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically   incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine   monoclonal antibody is used to identify cells that have incorporated BrdU.   This immunological detection scheme has several advantages over the use of   radioactive thymidine incorporation for identifying cells under-going   replication. Labeling and detection can be performed the same day instead of   waiting several days, as required for autoradiography of tritium-labeled   cells, and the necessity of using multiple specimens for obtaining the   optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine   staining with flow cytometric analysis allows multiple parameters to be   evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been   used for identi-fying proliferating cells in blood (Campana et al., 1988),   tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et   al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling   indices (Greipp et al., 1985). 
 
  Subcellular Location:
  Nuclear.
 
  SWISS:
  N/A
 
  CAS:
  59-14-3
 
  Important Note:
  This product as supplied is intended for research use only, not for use in   human, therapeutic or diagnostic applications. 
 
  增殖标志物
  BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。
  用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。