产品描述 概述CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant murine MIP-1α is a 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 71 % and 91 % amino acid sequence homology with the human and rat protein. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | | Purity | > 98 % by SDS-PAGE and HPLC analyses. | | Source | Escherichia coli. | | Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using murine splenocytes is in a concentration range of 10-100 ng/ml. | | Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. | | Endotoxin | Less than 0.1 EU/μg of rMuMIP-1α/CCL3 as determined by LAL method. | | Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. | | AA Sequence | APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA |
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