中文名称

8-羟基脱氧鸟苷/DNA/RNA Damage抗体

别    名

8 Hydroxyguanosine;   8-Hydroxy-2'-deoxyguanosine; 8-Hydroxydeoxyguanosine; 8 hydroxy 2’   deoxyguanosine; 8 hydroxyguanine; 8 hydroxyguanosine; 8 OHG; 8-OHG; 8OG;   8OHdG; 8OHG; 8 Oxoguanine; 8 hydroxyguanine; 8 Oxo 7,8 dihydroguanine; 8 oxo   Gua; 8 oxoG.  

抗体来源

Rabbit

克隆类型

Polyclonal

产品应用

ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
  not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user.

分 子 量

0.283kDa

细胞定位

细胞核 

性    状

Liquid or Solid

浓    度

1mg/ml

免 疫 原

KLH conjugated to 8-OHdG: 

亚    型

IgG

纯化方法

affinity purified by Protein A

储 存 液

0.01M TBS(pH7.4) with 1% BSA, 0.03%   Proclin300 and 50% Glycerol.

保存条件

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated   freeze/thaw cycles.

PubMed

PubMed

产品介绍

8-Hydroxydeoxyguanosine (8OHdG) is a   modified base that occurs in DNA due to attack by hydroxyl radicals that are   formed as byproducts and intermediates of aerobic metabolism and during   oxidative stress. There is increasing evidence to support the involvement of   free radical reactions in the damage of biomolecules that eventually lead to   several diseases in humans, such as atherosclerosis, cerebral and heart   ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation,   diabetes, aging, and neurodegenerative conditions, such as Alzheimer's   disease.
 
  Function:
  Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of   guanine. Guanine is the main target for reactive oxygen species in DNA, with   8-oxoguanine being the most frequent base lesion. Thus formation of   8-oxoguanine is an important biomarker of oxidative damage to DNA. It is   primarily repaired by the DNA glycosylase OGG1.
 
  SWISS:
  N/A
 
  CAS:
  3868-31-3
 
  Important Note:
  This product as supplied is intended for research use only, not for use in   human, therapeutic or diagnostic applications. 
 
  8-OHDG作为内源性及外源性因素对DNA氧化损伤作用的生物标志物，是一个极有前途的指标，通过8-OHDG的检测可以评估体内氧化损伤和修复的程度，氧化应激与DNA损伤的相互关系，对研究退行性疾病、衰老机制、癌发生机制、环境毒物与氧化应激的关系等均有重要的意义，也可以用来评价抗氧化剂治疗DNA氧化损伤的效果。已经证实，饮食、超氧化物歧化酶、Melatonin等对8-OHDG水平具有调节作用。近期日本学者制成一种抗氧化作用鸡尾酒，由葵花籽、绿茶和维生素c组成，证实了对FeC12诱导的8-OHDG异常增加有回调作用。随着研究的深入，8-OHDG的应用范围必将越来越广，但到目前为止，各种检测方法都还需进一步完善，因而深入开展这项研究工作将有十分重要的实际意义。