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产品信息 >> 试剂盒相关 >> ELISA 试剂盒;
Human GDNF

Human GDNF Quantikine ELISA Kit Summary

Assay   Type

Solid   Phase Sandwich ELISA

Format

96-well   strip plate

Assay   Length

4.0   hours

Sample   Type & Volume Required Per Well

Cell   Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL),   Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.696   pg/mL

Assay   Range

4.7 -   300 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum,   Plasma, EDTA Plasma, Heparin Plasma)

Specificity

Natural   and recombinant human GDNF

 

Cross-reactivity

<   0.5% cross-reactivity observed with available related molecules.< 50%   cross-species reactivity observed with species tested

Interference

No   significant interference observed with available related molecules.

Product Summary

The Quantikine® Human GDNF Immunoassay is a 4.0 hour solid phase ELISA designed to measure human GDNF levels in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant human GDNF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human GDNF showed linear curves that were parallel to the standard curves obtained using the Quantikine® Human GDNF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human GDNF.

Preparation and Storage

Shipping

The   product is shipped at ambient temperature. Upon receipt, store it immediately   at the temperature recommended below.

Storage

Store   the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDNF

GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.

Long   Name:

Glial   Cell line-derived Growth Factor

Entrez   Gene IDs:

2668   (Human); 25453 (Rat)

Alternate   Names:

Astrocyte-derived   trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic   factor; glial cell line derived neurotrophic factor; glial cell line-derived   neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1.     Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2.     Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3.     Add 50 μL of Assay Diluent to each well.

4.     Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5.     Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6.     Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7.     Aspirate and wash 5 times.

8.     Add 100 μL Substrate Solution to each well.

9.     Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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